Human Liver Proteome Project
Overview of Project
The overall aim of this project is to: generate an integrative approach that will lead to a comprehensive functional map of the liver; expand the liver proteome to its "PHYSIOME" and "PATHOME" in order to accelerate the development of liver-specific diagnostics and therapeutics; and develop standard operating procedures (SOPs) that will be applied to this and all other HUPO Initiatives.
The SOPs for collection, preparation and distribution of liver samples were established. Using the established SOPs, Prof. He and collaborators accepted samples from collaborators in both France and China and expression profiling was performed. In all, 4975 unique proteins and 2338 groups with 9245 proteins involved were identified from the French liver tissue samples. The transcriptome of human liver tissue, with 15426 genes, has been established and analyzed, in which 11806 are known genes and 3620 are EST. The bank for the ORFs has been established and there are more than 2700 full-length ORFs collected. A crude PPI network has been drafted, with 1291 proteins involved where the value is higher than 90. In addition, a murine hybridoma cell bank against human liver and plasma proteins, using unknown and native proteins as the immunogens, has been established. Among them, more than 1000 monoclonal antibodies reacted specifically with the 100 most highly abundant proteins in human liver and plasma. Meanwhile, in June 2005, at the HLPP workshop organized by Dr. Beretta, a subproject focused on Hepatocellular Carcinoma (HCC) was launched.
Collaborations with other Initiatives
- Proteomic standards, data formats and data mining are coordinated with the Proteome Standards Initiative.
- Cooperating with the Human Antibody Initiative to develop a HLPP Antibody banking subproject.
- Integrating data analysis activities with the Human Plasma Proteome Project.
HLPP Newsletters
Key Documents Related to Activities
- HLPP August 2008 workshop Announcement
- HLPP Bethesda Workshop Summary July 2003
- HLPP Bethesda Workshop Recommendations for US participation July 2003
- Liver Proteome Initiative Workshop (July 17-18, 2003, Bethesda) : Final Agenda
- HLPP Update-May 2003
- Proposal and Survey Form
- HLPP report August 2004
- CNHLPP start-up-Nov. 2004 (coming soon)
- The summary of the workshop of the HLPP disease sub-project -June 2005 (coming soon)
- HLPP report July 2005
- CNHLPP report-July 2005 (coming soon)
