Péter Horvatovich, University of Groningen, Netherlands

Guest Editors

Young‐Ki Paik, Yonsei University
Eric Deutsch, Institute for Systems Biology
Fernando Corrales, Centro Nacional de Biotecnología (CSIC)
Lydie Lane, Swiss Institute of Bioinformatics
Gilbert S. Omenn, University of Michigan

Associate Editor

Christopher M.Overall, The University of British Columbia

Thematic Priorities:

• Completing the high-resolution draft of the human proteome with new strategies and results leading to confident identifications of neXtProt missing proteins (PE2 – 4) according to the C-HPP Guidelines v 2.1 or recent updates
• Progress on the protein list of individual chromosomes and groups of chromosomes, annotating known proteins and their isoforms/proteoforms and/or credibly identifying missing proteins (PE2 – 4)
• Annotating proteins and their isoforms/proteoforms and/or identifying missing proteins found in rare or under explored cells and tissues, and protein lists of human cell types as a step in creating a human cell proteome atlas
• Produce and utilize “popular proteins” lists in B/D-HPP and contribute to the identification of missing proteins
• Proteomic studies of proteoforms produced by proteolytic processing, PTMs, alternative splicing (ASV),
• coding non‐synonymous single nucleotide polymorphisms (cSNPs), or chromosome abnormalities
• Use of targeted proteomics, especially SRM and MS‐SWATH, to extend chromosome‐based protein findings
• Disease studies utilizing chromosome information, characterizing amplicons, cis‐regulated pathways or networks
• New bioinformatic tools and approaches for annotating the human proteome
• Biological mechanistic analyses inspired from proteomics data in diseases or biological processes
• Biomarker discoveries based on the identification of novel ASVs, PTMs or cSNPs in proteomic studies

Submission Procedure

Manuscripts must be submitted by 31st May, 2019 to be considered for this Special Issue. Manuscripts must be submitted electronically through the ACS Paragon Plus Environment online submission system. Specify in the authors’ cover letter that the manuscript is intended for the HPP Special Issue.

Review and Publication Process

HPP Data Guidelines

Papers must conform to both the Journal of Proteome Research mass spectrometry guidelines and the HPP guidelines v 2.1 (see Deutsch et al. http://pubs.acs.org/doi/abs/10.1021/acs.jproteome.6b00392) in order to be sent to review and for acceptance. Please check for any changes to the HPP guidelines available online at http://www.thehpp.org/guidelines/ before submission. All papers must analyze their data using the Human PeptideAtlas release 2019-01 and neXtProt release 2019-02. Papers not doing so will be returned without review.

21st C-HPP symposium “Illuminating the Dark proteome” - Saint Malo, France, May 12-14, 2019

The central theme of the 21st C-HPP symposium is to discuss approaches and topics on “Illuminating the Dark proteome”, a colloquial term that includes missing proteins (PE2 – PE4), uncertain/dubious predicted proteins (PE5), uPE1 proteins, smORF (small proteins), and any proteins translated by long non-coding RNAs or uncharacterized transcripts including those arising from non-coding regions of DNA and/or novel alternative splicing. The symposium will also welcome speakers from the B/D-HPP to discuss the functionalization of the dark proteome.

Saint-Malo, historical home of great discoverers and corsairs, is a fortified city located on the north coast of Brittany. The symposium will take at the Hotel France & Chateaubriand, which is located inside the “stone vessel” (the nickname of the fortified city). Registration for the symposium can be done online using a registration form that must be sent directly to the hotel. We are pleased to announce that the deadline of abstract submission has been extended up to March 11. Please visit https://c-hpp.univ-rennes1.fr/ for more details.

For sure, the wonderful location and relaxing atmosphere of Saint-Malo will allow participants of the symposium to exchange fruitfully and informally. Yet, spring has arrived in the Corsair City…

A message from Cabezon Group and Clarivate Analytics

The National Cancer Institute’s (NCI) Office of Cancer Clinical Proteomics Research (OCCPR) has contracted with Cabezon Group and Clarivate Analytics to conduct a third-party, external evaluation which includes a research survey of the external perception of the Clinical Proteomic Tumor Analysis Consortium (CPTAC) program. The survey is designed to be relatively short and should only take approximately 10 to 15 minutes to complete. To complete the survey, please click on https://www.surveymonkey.com/r/CPTAC_HUPO.

Your strictly voluntary and confidential participation in the survey is vital to our evaluation efforts. The goals of the survey are to examine the impact of CPTAC on the broader field of proteogenomics; how the proteogenomic research community perceives the CPTAC program; and to assess the impact that specific CPTAC program components and actions have had on the research community. Your feedback provides a collaborative, shared understanding of program perceptions which enables improvements intended to benefit future CPTAC activities and impact.

The research results from the evaluation and survey will be disseminated publicly. A summary of the survey results will be placed on the Office of Cancer Clinical Proteomics public website (proteomics.cancer.gov). In addition, the survey results will be presented at the 2020 annual CPTAC meeting.

You may forward the survey invitation/link to colleagues, but we ask that there is no addition or modification to the invitation message. If you have already completed this survey from another list serve we ask that you do not complete it again.

Thank you for your time and participation.

Vera Ignjatovic,University of Melbourne, Australia

Glycoproteomics holds immense potential to advance understanding of the molecular and cellular processes underpinning human (patho)physiology, but remains an analytically challenging discipline (Thaysen-Andersen M et al., Mol Cell Proteomics. 15(6):1773. 2016). Accurate automated intact glycopeptide identification from high resolution tandem mass spectrometry data, a prerequisite for the further advancement of glycoproteomics, is still in its infancy despite many exciting glycoinformatics developments (Hu et al., Mass Spectrom Rev. 36(4):475. 2017).

Formed in September 2016, the first community study of the B/D-HPP Human Glycoproteomics Initiative (HGI) sets out to provide a detailed comparison of the bioinformatics solutions currently in use for the site-specific determination of protein N- and O-linked glycosylation. For this purpose, two high resolution CID-, HCD- and EThcD-based LC-MS/MS glycopeptide datasets were acquired from a tryptic digest of serum glycoproteins and these two common data files were distributed to all study participants. The participating teams were asked to return lists of confidently identified N- and O-glycopeptides from the two LC-MS/MS data files using preformed reporting templates and, further, to provide information of their used method of identification. Significant efforts by the HGI study committee were invested to ensure uniformity and compliance of all teams with the study guidelines.

An update of the study progress was presented by Dr Thaysen-Andersen at the B/D-HPP Investigator Meeting during the 17th World Congress HUPO, Orlando, FL. The study has been very well received by the community with 22 glycoproteomics laboratories encompassing 9 software developers and 13 user teams located across all major continents completing the identification task by the end of the reporting deadline (October 2018). Recent analysis of the glycopeptide data reports by the HGI study committee encouragingly shows the existing of diverse informatics tools and approaches with a great potential for (semi-)automated glycopeptide identification from complex LC-MS/MS datasets, but, at the same time, highlighted a significant variance of glycopeptide/protein identifications within and between the user and developer teams. Discordance was also observed between participants using the same software. The underlying reasons for this spread and further data comparisons to delineate the more exact overlap and differences between the glycopeptides reported by the 22 teams are currently being investigated.

We aim to publish the 1st HGI study results in 2019 and will present the final study outcomes at the 2nd Australasian Glycoscience Symposium (2nd AGS) to be held on the 14th September in Adelaide, Australia (more information to follow) immediately prior to the 18th World Congress HUPO, Adelaide, Australia where the prospects and scope of a potential 2nd HGI study also will be discussed.

Péter Horvatovich, University of Groningen, Netherlands

The dark proteome is defined accordingly to C-HPP as “a colloquial term that includes missing proteins (PE2 – PE4), uncertain/dubious predicted proteins (PE5), uPE1 proteins, smORF (small proteins), and any proteins translated by long non-coding RNAs or uncharacterized transcripts including those arising from non-coding regions of DNA and/or novel alternative splicing.” The central theme of the 21st C-HPP symposium is to discuss approaches and topics on “Illuminating the Dark proteome” and will take place in May 12-14, 2019 in Saint Malo (France) at the Hotel France & Chateaubriand, which is located inside the fortified city. The program is currently under construction and will be placed online either on the home page of the event or at C-HPP Wiki soon. Registartion for the workshop can be performed online using a registration form. The organisers Charles Pineau, Chris Overall, Young-Ki Paik, Lydie Lane are looking forward to receive all C-HPP members and all interest participants. Please visit https://c-hpp.univ-rennes1.fr/ for more details.

Vera Ignjatovic,University of Melbourne, Australia

Gil Omenn is one of the founding members of the Human Proteome Organization, has led the Plasma Proteome Project from 2002 through 2010, and chaired the Human Proteome Project from 2010 through 2018. He also served as President of the U.S. HUPO. Gil is a Distinguished University Professor of Computational Medicine & Bioinformatics, Internal Medicine, Human Genetics, and Public Health at the University of Michigan and a member of the U.S. National Academy of Medicine.

Human Proteome Project

What would you like to see happen in the HPP over the next 3 years?
Gill: I would like to see a coalescence of subsets of the C-HPP teams and subsets of the B/D-HPP teams to accelerate scientific progress on the overriding aims of the HPP: (a) completing and annotating the protein parts list and (b) making proteomics an expected part of all multi-omics studies in biomedical research. I am keen to see the four resource pillars drive collaborative studies across antibody profiling, mass spectrometry, bioinformatics, and pathology. And I would like to see significant growth in numbers of participants in the HPP and the HUPO World Congress.

What do you see as the role of the HPP in the future progress of proteomics? Gill: The HPP has worked openly with proteomics researchers globally, with an emphasis on quality of data, sharing of complete datasets and metadata, and metrics for the annual progress on the “draft human proteome”. I hope we will see much more extensive application of quantitative proteomics and systems biology across all the fields of medical research.

Do you see the HPP Knowledgebase as critical for the future of the HPP? Gill: Yes, indeed. The combination of neXtProt, PeptideAtlas, ProteomeXchange/PRIDE, and interchange with other resources internationally has greatly accelerated the field of proteomics. Data Quality Guidelines for Mass Spectrometry and the International Working Group on Antibody Validation are critical for credibility and progress in the field and in the HPP. Reliable, fully shared and reconfirmed data with tools like the TransProteomicPipeline, neXtProt uniqueness mapper, the Universal Spectra Identifier, and the SRM Atlas and Popular Proteins lists can accelerate biological and clinical applications from the HPP and the community at large.

Science 

What is your advice to early career scientists? Gill: I am proud of the significant commitment to Early Career Scientists in the development of the HPP and especially the B/D-HPP. I urge young colleagues to ask major questions about the field of science they choose, to be alert for relevant stimulation from other fields of science, to connect research with unmet needs in clinical medicine, and to master the technical aspects of their scientific work. Always make an effort to build personal relationships and find ways to enjoy your chosen work. Make sure your potential mentors are up-to-date in the field and able to provide cogent guidance on the research you hope to undertake.

Where do you see the gaps in technologies? Gill: One of the persistent gaps is the separate use of antibody profiling and mass spectrometry; we need studies that deploy both approaches on the same specimens. In the field of epigenetics, there is a chasm between analyses of DNA methylation and analyses of histone marks. The latter is often neglected by the genomics folks. This requires links between DNA and protein sample prep and analytical methods and the researchers. There is a similar gap between evidence of splice isoforms in the RNA-Seq transcript data and in direct studies of proteins. Alternative splicing is one of the most remarkable features of the evolution of multi-cellular organisms, with their intron/exon gene structures. It is shocking that many researchers lump together the splice isoforms from the same gene and presume that they are functionally the same; in many cases, we know that is not true. Finally, single-cell transcriptomic and CyTOF proteomic studies are proving feasible and valuable in cancer biology and developmental biology.

What do you see would be the role of proteomics in the clinic? Are devices such as MasSpec Pen possible as future implementations in the clinical routine? Gill: We need high throughput, reasonably sensitive, moderate cost assays combined with specific biomarkers to generate actionable data for clinical decisions and for epidemiologic studies, as well. There has been progress, especially in Europe, with MS for microbial diagnoses and inherited disorders in clinical settings.

The MasSpec Pen has generated a lot of press attention during the past 2-3 years. Articles from Hungary in 2013 and from University of Texas/Austin in 2017 in Science Translational Medicine have reported an automated, biocompatible handheld mass spectrometry device for rapid, non-destructive diagnosis of human cancer tissues. The MasSpec Pen enables controlled, automated delivery of a discrete water droplet to a tissue surface for efficient extraction of biomolecules within a few seconds, which is then delivered to the mass spectrometer for molecular analysis of proteins and metabolites. The usefulness depends on the quality of the cancer biomarkers assayed and the rapidity of analysis. The device is still in the clinical development pathway with results reported from studies on mice and on cells in vitro. I have no experience with this device, which may not yet be FDA-approved. The concept for its use in surgical removal of cancers is to sample the margin of the surgical field and rapidly analyze a few key proteins in the hand-held device.

Personal

What/Who was the largest influence in you moving your career into proteomics? Gill: I became interested in proteins as an undergraduate at Princeton; actually, I had problems growing and isolating sufficient protein from the acellular slime mold Physarum polycephalum, so I ended up doing electron microscopy of its previously unrecognized cytoplasmic latticework! At Harvard Medical School with a young faculty member Tom Gill III and during a summer research experience at The Weizmann Institute of Science with the inspirational Ephraim Katzir (later the President of Israel), I worked on synthetic polypeptides as models of proteins. Then, for my military duty during the Vietnam War, I had a wonderful opportunity to work at NIH with Christian B. Anfinsen on structure and function studies of staphylococcal nuclease. He received the Nobel Prize in 1972 for the concept and the compelling evidence that the amino acid sequence of proteins determines the 3-dimensional folding and function of each protein. After decades of work in human behavioral genetics and eco-genetics, and leadership roles in government, public health, and healthcare, I returned to proteins in collaboration with Sam Hanash with the founding of the Human Proteome Organization in 2002. For those interested in my personal journey, here is a link to my U of Michigan Distinguished University Professor Lecture about “Paths Less Travel’d” (a phrase from Robert Frost, who taught at the U of M), video link to my DUP lecture.

What was the most exciting scientific finding of your career? (The eureka moment!) Gill: Clinical: As a 4th year medical student, the recognition of a syndrome of primary immune deficiency affecting 12 children in one large kindred, later named “Omenn syndrome” by McKusick of Johns Hopkins and then cured by bone marrow transplantation by a French team and linked to Rag1/Rag2 genes.

Mechanisms: The recognition that cancers of non-endocrine organs, like lung, sometimes produced hormonal disorders not due to “disordered protein synthesis” but by activation or de-repression of the corresponding gene for the polypeptide, which is available in every cell type and would yield exactly the same sequence as the normal hormone.

Public Health: The shocking finding that the combination of beta-carotene and retinyl palmitate, hypothesized to prevent lung cancers and cardiovascular deaths, actually caused more cancers and more CVD deaths in our beta-Carotene and Retinol Efficacy Trial (CARET).

How do you keep a work/life balance? If there is such a notion? Gill: Yes! I have always enjoyed my studies and my work, as I do currently, while pursuing a variety of interests that broadened my social interactions and made for a stimulating life. I played clarinet in the band, oboe in the orchestra, sax in the dance band, and piano lots of places (still do). I played intramural tennis at Princeton and Harvard Medical School, and remain competitive in doubles tennis. I have long been active in science policy matters and political campaigns, as well as serving in the federal government as a White House Fellow at the Atomic Energy Commission, as associate director of the White House Office of Science & Technology Policy and the Office of Management & Budget, chairing the Presidential/Congressional Commission on Risk Assessment and Risk Management, and serving on the Scientific Management Review Committee for the NIH, as well as boards of cultural organizations. My wife, Martha Darling, whom many of you have met, also has a wide range of national and international activities, which we often enjoy together.

What do you consider the major achievements of the HUPO Human Proteome Project (HPP)?

Gill: 1. The HPP commitment to high quality science in proteomics experiments and data analysis. This includes the development and wide utilization of the HPP Guidelines for Interpretation of Mass Spectrometry Data (v2.0, JPR 2016) and the encouragement of generations of improvements in the underlying technology platforms.

2. The placement of neXtProt, PeptideAtlas, and Human Protein Atlas at the center of the proteomics world.

3. The creation of ProteomeXchange in conjunction with the European Bioinformatics Institute and PRIDE to register and make widely available the primary data and meta-data from all proteomics experiments, incorporated into the HPP Guidelines.

4. The organized effort to apply standardized reanalysis of all MS-based proteomics datasets at PeptideAtlas and curation of multiple kinds of protein studies at neXtProt to progressively complete the “protein parts list” for the human proteome. As of January 2018, 87% of predicted protein-coding genes (17,470 proteins) were rated as having protein evidence PE1 (of a total of 19,656 PE 1+2+3+4). This percentage continues to rise, with leadership from the Chromosome-centric C-HPP and the KnowledgeBase resource pillar of the HPP. Each year the HPP published the “Metrics paper” in the Journal of Proteome Research with progress from the entire community.

5. The development of the SRM Atlas to expedite targeted proteomics for quantitative biological studies throughout the life sciences by providing spectra from synthesized, predicted proteotypic peptides of nearly every predicted protein.

6. The use of bibliometric analyses to identify the most investigated proteins (“popular proteins”) by organ and disease category and connect those research communities to the SRM Atlas as a guide to quantitative targeted assays of those key proteins and their pathways and networks, led by the Biology and Disease-based B/D-HPP.

7. The tissue-specific and intracellular localization of expression of proteins with immunohistochemistry/ immunofluorescence, correlation with organ-specific transcript expression, and quality assurance of antibody specificity by Human Protein Atlas (Antibody-Profiling resource pillar of the HPP).

8. Continued effort to make proteomics an obligatory component of multi-omics research protocols, recognizing that crucial properties of proteins—dynamics of abundance, post-translational modifications, and splice isoform variants—cannot be predicted or detected at the gene or transcript levels. Complemented by the C-HPP-led initiative to find evidence of function for the 1260 PE1 proteins lacking specific functional annotation.

9. Multiple efforts to engage, mentor, and highlight research from Early Career Researchers, the future of the field.

Happy New Year!

On behalf of all of the HUPO Executive Committee I’m delighted to be able to wish all HUPO members a very happy and healthy 2019. I hope your year ahead will be productive and enjoyable. Having had a successful 2018, HUPO has much to look forward to in the coming year.

In my role as the incoming President, my main objective is to work inclusively with all the HUPO committees and members to raise awareness of the impact of proteomics. Impact that arises from the significant advances that have been made recently and from those that will undoubtedly emerge in 2019. I hope collectively we can work to ensure that the central importance of proteomics in our understanding of human biology – in health and disease - is recognized at all levels - from the public, to research funders and policy makers. I’m looking forward to working with you all to achieve this and if there is anything that I, or HUPO, can do to help you in your research endeavors and career in proteomics then please don’t hesitate to get in contact.

Of course, I’m also looking forward to meeting as many existing and new individual HUPO members at conferences and other events throughout 2019 but especially at our flagship meeting, HUPO 2019, in Adelaide this coming September (www.hupo2019.org). I encourage you to participate in HUPO 2019 as it will undoubtedly be a superb forum for presenting and discussing your research.

As you are hopefully aware, the Human Proteome Project (HPP) is HUPO’s flagship project. Through the quite remarkable leadership of Gil Omenn, the HPP has coordinated and been responsible for many significant achievements – far too many to document here but to be found in previous issues of HUPOST. Through Gil’s hugely impressive and dedicated efforts the HPP is now firmly established as the central hub for HUPO’s research activities and under the new leadership of former HUPO President Mark Baker is poised to develop further. 2019 is a great time to get involved in the HPP and I encourage you to find out what it is doing and get involved.

Finally, conscious of the successes HUPO has achieved through the efforts of many individuals - too many to mention and thank – I would like to acknowledge the contribution made by our most recent past President – Mike Snyder. Of Mike’s many impressive HUPO achievements perhaps most notable and quite unique has been the hPOP initiative. Don’t know what that is? Then again I encourage you to be proactive, find out and get involved!

I’m sure you will find much in this issue of HUPOST, please enjoy!

My message for 2019: HUPO is an international and inclusive team that actively welcomes your participation - be proactive, get involved.

Michelle Hill, QIMR Berghofer Medical Research Institute, and The University of Queensland, Australia

Congratulations and thank you for accepting the role of B/D-HPP HUPOST  cordinator.

Thank you, Michelle. It is an honour to be suggested for this position and to contribute to showcasing the excellent efforts of the BD-HPP.

Q. Firstly, can you please teach the proper pronunciation of Ignjatovic?

Congratulations on spelling my surname correctly! The pronunciation of my surname is problematic for many people, so they avoid trying to pronounce it at all. All I can say is that it appears harder than it is.

My surname is Serbian. This is a country where I was born and where I spent my early childhood, until my parents decided to move across the World, to Australia, exactly 30 years ago.

Q. Can you please explain about your research?

My research focuses on age-specific differences, the developmental differences between neonates, children and adults. These differences are described by the concept of Developmental Haemostasis, when focusing on the blood clotting system; or a concept of Developmental Proteomics when it comes to the proteome in general. In terms of proteomics, I am using plasma as a system to study how protein expression and post translational modifications change with age, how these changes can be applied to early disease detection and their implications for therapeutic approaches in these populations.

Q. How did you become involved in B/D-HPP?

In 2013 I was approached to be one of the two funding members of the PediOme initiative. That was the start of an adventure that has taken me deep into the B/D-HPP and across other aspects, such as the PPP.

Q. What was your motivation in starting the Human Pediome Initiative?

The motivation behind the PediOme initiative was a clear lack of pediatric studies, which meant that we had a very limited knowledge of the proteome in children (including newborns and infants). What was particularly missing was the knowledge of the “healthy state”, without which early detection of disease or individuals susceptible to specific diseases simply can’t happen. There are a couple of teams internationally that are making good progress in closing these knowledge gaps, but, there is still a lot of work to do.

I would like to take this opportunity to urge anyone who is interested in becoming a part of the PediOme initiative to contact me, as we need people who want to drive progress in pediatric proteomics.

Q. What do you see as the next challenges for HPP and B/D-HPP?

The HPP has made important progress since 2010 and has brought proteomics much closer to the clinic. I believe that the next challenges for the HPP and B/D-HPP will be standardization of methodological approaches, such that they can be applied to multi-site international clinical laboratories for the purpose of diagnosis, not research.

Q. Would you like to share some of your interests outside of science?

Science is a big part of my life, but, I ensure that I always find the time to do things that take me away from science…always good for getting a different perspective…things such as camping, spending time with family and friends, reading, cycling. I have recently crossed the finish line in my first ever marathon, the Melbourne marathon, and that is an achievement that I am extremely proud of.

The 18th Human Proteome Organization World Congress - HUPO 2019 will be hosted by the Australasian Proteomics Society (APS) in the beautiful ‘City of Churches’, Adelaide. HUPO 2019 will focus on “Advancing Global Health Through Proteome Innovation” and will bring together world-leading experts and the next generation of early career scientists to promote how proteomics is advancing our knowledge of human and planetary health. HUPO 2019 will both celebrate what has been achieved and look forward to future advances and discoveries that will revolutionize global health.

The Australasian Proteomics Society (APS) has started to design a fantastic program for HUPO 2019 in Adelaide and have confirmed the following plenary speakers:

• Prof. Ruedi Aebersold
• Prof. Yu-Ju Chen
• Prof. Fuchu He
• Prof. Albert Heck
• Prof. Kathryn Lilley 
• Prof. Mathias Uhlén

With plenary lectures from outstanding speakers and over 30 parallel sessions - including 6 HPP sessions, the 2nd Australasian Glycoscience Symposium, Plant and Food Proteomics sessions - the program will offer opportunities for fruitful discussions. Other Themes include:

• Health and Disease
• Biological Applications of The Proteome
• Beyond the Proteome
• Our Human Environment
• Enabling Technologies

The Organizing Committee of the HUPO 2019 consists of the committee members of the Australasian Proteomics Society (APS) plus a number of prominent Australian Proteomics Scientists:

HUPO 2019 Organizing Committee

Prof. Stuart Cordwell (Co-Chair, APS President)
Prof. Peter Hoffmann (Co-Chair and LOC Chair, APS Vice President))
Prof. Anthony Purcell (APS Treasurer)
Prof. Mark Molloy (APS Secretary)
Prof. Marc Wilkins (APS Committee)
Dr. Michelle Colgrave (APS Committee)
Dr. Morten Thaysen-Andersen (APS Committee)
Dr. Andrew Webb (APS Committee)
Additional HUPO 2019 Organizing Committee
Prof. Mark Baker (HPP)
A/Prof. Michelle Hill (HUPO EC Secretary)
A/Prof. Vera Ignjatovic (HPP)
Prof. Ed Nice (HPP and social event committee)
Prof. Nicolle Packer (Glycoscience)
Prof. Paul Haynes (Plant and Food Proteome)
Prof. Gavin Reid (Multi-omics)

Visit the conference website www.hupo2019.org for more details.

We look forward to welcoming you to Adelaide in 2019 for an unforgettable HUPO World Congress.

Sanjeeva Srivastava, IIT Bombay, India

Proteomics is the study of entire protein complement of an organism. Advancements in proteomics have been phenomenal over the last decade with several promising high-throughput technologies emerging at the forefront of various applications. Owing to the rapid advancements in state-of-the-art proteomics technologies, continuous expansion of our scientific understanding, and challenges associated with omics research, it has become essential to keep up with current trends and advances in proteomics research. In this light, we conducted three workshops at IIT Bombay, where eminent scientists and researchers from India and abroad had shared their knowledge and expertise to train the participants and familiarize them to the vast applications of proteomics.

Basics and Advanced Proteomics Approaches (December 3 to 14, 2018)

The DST supported programme was specially designed for Scientists/Technologists who are actively involved in research and development activities. The overall goal of the programme was to develop an educational training program on diverse proteomic technologies and offer hands-on training on the cutting edge proteomics technology to benefit the scientists in their research skill enhancement and keeping them abreast with the last technological advancements in the field of science. 25 scientists were selected for this program.

Cancer Proteogenomics Workshop (December 6 to 11, 2018)

This training program utilized advanced genomic & proteomic technologies and their data from high-quality human biospecimens to identify potentially actionable therapeutic molecular targets. This IUSSTF funded training program was a collaborative effort by experts in the fields of proteomics and proteogenomics in cancer research from the Broad Institute of MIT and Harvard (Convener, USA: D. R. Mani) and Indian Institute of Technology Bombay (Convener, India: Sanjeeva Srivastava). The program comprised interactive lectures with case studies, hands-on sessions and demonstrations on proteogenomics aimed at accelerated understanding of cancer. 200+ participants attended this training program.

Caner proteogenomics workshop Group 

Cancer Moonshot India Program – December 7, 2018

The Cancer Moonshot aims to accelerate cancer research to make effective therapies available to more patients, while also improving early and accurate detection.

https://www.cancer.gov/research/key-initiatives/moonshot-cancer-initiative

Similar to the Cancer Moonshot project of USA, to expedite cancer diagnosis and treatment, we have initiated the Cancer Moonshot India project. India has now become the 12th country to join the International Cancer Proteogenome Consortium (ICPC) of the National Cancer Institute (NCI), U.S. The Indian Institute of Technology Bombay (IITB) aims to study proteomics and the Tata Memorial Hospital (TMH), Mumbai, aims to study the genomics of three cancers, breast, cervix and oral. Dr. Henry Rodriguez, Director, NCI Office of Cancer Clinical Proteomics Research inaugrated this event and delivered a speech to the gathering, which included distniguished clinicians from ACTREC, TMC and KEM hospitals; key industry leaders; and scienitsists working in genomics and proteomics area.

Enchanting dance performances by Faizal Kahan and his group

Trans-Proteomic Pipeline (December 12 to 14, 2018)

The TPP workshop was scheduled from 12th to 14th December 2018. This advanced mass spectrometry data analysis workshop was supported by IUSSTF joint virtual center grant and conducted by Institute for Systems Biology scientists.

HUPO President (2017-2018), Mike Snyder, California, USA

It has been a true honor to have served as HUPO President for the past two years. The field of proteomics has never been more exciting with numerous advances in technology, transformational research findings, and improved integration into systems biology and medicine. We can now profile thousands of proteins at rapid speed and with instrument sensitivities that enable single cell proteomics. Through the efforts of the Human Proteome Project (HPP) the number of uncharacterized proteins has rapidly diminished (to under 3,000) and the Human Protein Atlas has mapped the expression and subcellular localization of the majority of human proteins. Our annual HUPO meetings continue to present the very best science in proteomics and attract the very best practitioners of our field. Most importantly, we have welcomed many new scientists at our annual meeting and benefited from their outstanding contributions on numerous committees. We have undertaken many bold initiatives such as the launch of the international Human Proteomics Moonshot project. The international influence and stature of the organization is evidenced by the enthusiastic participation of former US Vice President Joseph Biden at our annual meeting. The HPP continues to make great progress as our flagship project, with Mark Baker assuming the helm after the Herculean efforts of Gil Omen to get the HPP launched and running smoothly.

Despite our many successes, there remains much to be done and we cannot afford to be complacent—this is the same as falling behind. We still cannot profile all proteins and isoforms with low sample amounts and with high throughput. Depth and speed will be important for large studies, similar to other omics fields. Single cell proteomics is promising but still in its infancy. As leaders in the field, HUPO members need to educate our peers and funding agencies about the significant value of incorporating proteomics into all major projects going forward. Many researchers and some funding agencies are beginning to recognize that understanding biological systems requires proteomics, which is closer to phenotypes than nucleic acid assays, and this has shaped the scope of some newer initiatives, for example the MoTrPAC consortium. Finally, I believe that medicine of today using large sample volumes and visits in the clinic will be replaced by small “finger prick” sample collection and mail-in assays. HUPO and the field of proteomics needs to be at the forefront of this. I anticipate major advances in the field of proteomics in the coming years and believe HUPO will play a pivotal role in making these happen. I think we are in terrific hands under Steve Pennington’s leadership. My heartfelt thanks for everyone‘s contributions during my term.