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Biology-Disease Human Proteome Project (B/D-HPP) Hot Papers

05 Jun 2017 11:15 AM | Deleted user

1. Human Brain Proteome Project (HBPP)

Garcez et al. Sci Rep. 2017 Jan 23;7:40780. doi:10.1038/srep40780. Zika virus disrupts molecular fingerprinting of human neurospheres.

Combining proteomics and mRNA transcriptional profiling, we found over 500 proteins and genes associated with the ZIKV infection in neurospheres associated to impairments in the cell cycle and neuronal differentiation. Our results point to biological mechanisms implicated in brain malformations, which could be exploited as therapeutic potential targets to mitigate it.

2. Human Immuno-Peptidome Project (HIPP)

Abelin JG, Keskin DB, Sarkizova S, Hartigan CR, Zhang W, Sidney J, Stevens J, Lane W, Zhang GL, Eisenhaure TM, Clauser KR, Hacohen N, Rooney MS, Carr SA, Wu CJ. (2017). Mass Spectrometry Profiling of HLA-Associated Peptidomes in Mono-allelic Cells Enables More Accurate Epitope Prediction. Immunity 46:315-326.

HLA class I binding prediction has traditionally been based on biochemical binding experiments. Abelin and colleagues present an LC-MS/MS workflow and analytical framework that greatly accelerates gains in prediction performance. Key advances include the discovery of sequence motifs and improved quantification of the roles of gene expression and proteasomal processing. 

Workshop Report from 8th Workshop on Affinity Proteomics, Jochen Schwenk

The 8th Workshop on Affinity Proteomics was held in Alpbach, Austria from March 12th-15th. The meeting brought together more than 130 attendants and recent advances in the generation and use of binding reagents. A broad range of applications were presented including expanded use of microarray and immunoassay systems, as well as mass spectrometry based assessment of antibody selectivity. A panel of researchers, reagent providers and journal editors then discussed the challenges and opportunities of the field today and how to address these for the years to come. 

This included the need to: 

  • validate the affinity reagent in and for a specific application and sample context
  • provide transparent information about validation (e.g. accessibility to primary data)
  • collect trackable information about affinity reagent (e.g. origin, LOT and product number, clone or sequence)
  • standardise the assessment criteria and assays

The 9th Workshop on Affinity Proteomics is planned to be held in Alpbach in two years.

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