B/D HPP
Cardiovascular Initiative
GET INVOLVED: Get in touch at office@hupo.org to learn more
Mission & Goals
Cardiovascular diseases are a leading cause of mortality and morbidity in the world. Despite ongoing efforts, current options for diagnosis and treatment are limited, and the underlying molecular mechanisms that lead to the manifestation of detrimental phenotypes in the heart remain largely elusive. The major goals of the HUPO Cardiovascular Initiative are to develop and apply cutting-edge proteomics and other omics technologies to map the dynamic cardiac and vascular proteomes, elucidate cardiovascular disease mechanisms, identify candidate therapeutic targets, and provide clinically useful diagnosis as well as risk prediction.
Our Work
Emphasis is given to elucidating the complete cardiovascular proteome at single cell and proteoform resolution, and applying large-scale screening toward clinical diagnostics and prognosis of heart diseases.
We are currently working on:
Ai L, Binek A, Zhemkov V, Cho JH, Haghani A, Kreimer S, Israely E, Arzt M, Chazarin B, Sundararaman N, Meyer JG, Sharma A, Marbán E, Svendsen CN, Van Eyk JE. Single-Cell Proteomics Reveals Specific Cellular Subtypes in Cardiomyocytes Derived From Human iPSCs and Adult Hearts. Mol Cell Proteomics. 2025 Sep;24(9):100910. doi: 10.1016/j.mcpro.2025.100910. Epub 2025 Jan 22. PMID: 39855627; PMCID: PMC12445721.
Buck KM, Rogers HT, Gregorich ZR, Mann MW, Aballo TJ, Gao Z, Chapman EA, Perciaccante AJ, Price SJ, Lei I, Tang PC, Ge Y. Extracellular matrix alterations in chronic ischemic cardiomyopathy revealed by quantitative proteomics. JCI Insight. 2025 Sep 30;10(21):e196933. doi: 10.1172/jci.insight.196933. PMID: 41026521; PMCID: PMC12643490.
Di Paola M, Reitz CJ, Kuzmanov U, Jia K, Gramolini AO. Subcellular proteomic analyses reveal REEP5 knockdown in the mouse heart disrupts mitochondrial networks. Mol Cell Proteomics. 2026 Feb 9:101527. doi: 10.1016/j.mcpro.2026.101527. Epub ahead of print. PMID: 41672147.
Han Y, Binti S, Wennersten SA, Pandi B, Ng DC, Lau E, Lam MP. A ratiometric catalog of protein isoform shifts in the cardiac fetal gene program. JCI Insight. 2025 Aug 7;10(18):e184309. doi: 10.1172/jci.insight.184309. PMID: 40773290; PMCID: PMC12487847.
Subramanian SP, Wojtkiewicz M, Yu F, Castro C, Schuette EN, Rodriguez-Paar J, Churko J, Renavikar P, Anderson D, Mahr C, Gundry RL. Integrated Multiomics Reveals Alterations in Paucimannose and Complex Type N-Glycans in Cardiac Tissue of Patients with COVID-19. Mol Cell Proteomics. 2025 Apr;24(4):100929. doi: 10.1016/j.mcpro.2025.100929. Epub 2025 Feb 22. PMID: 39988192; PMCID: PMC12131855.
Cardiovascular Initiative Membership
Come join us! Get in touch and let us know if you are interested in joining our initiative!
Leadership Information
Chair:
Maggie Lam, PhD
University of Colorado, Aurora, CO, USA
Chair:
Rebekah L. Gundry, PhD
University of Nebraska Medical Center, Omaha, NE, USA
Past Chairs:
Peipei Ping, PhDUniversity of California, Los Angeles, CA, USA
Jennifer E. Van Eyk, PhD Cedars-Sinai Medical Center, Los Angeles, CA, USA
Executive Committee Members:
Justyna Fert-Bober, PhD Cedars-Sinai Medical Center, Los Angeles, CA, USA
Ying Ge, PhD University of Wisconsin-Madison, USA
Anthony Gramolini, PhD University of Toronto, Ontario, Canada
Jonathan Kirk, PhD Loyola University Chicago, IL, USA
Edward Lau, PhD University of Colorado, Aurora, CO, USA
Merry Lindsey, PhD University of Nebraska Medical Center, Omaha, NE, USA
Manuel Mayr, MD, PhD King’s College London, London, UK
Mary Papadaki, PhD Loyola University Chicago, IL, USA
Melanie White, PhD University of Sydney, Sydney, Australia
Early Career Researcher:
Elizabeth Bayne, BA University of Wisconsin-Madison, USA
Linda Berg Luecke, MS University of Nebraska Medical Center, Omaha, NE, USA
Thomas Martin, BS University of Colorado, Boulder, USA
Sabari Subramanian, PhD University of Nebraska Medical Center, Omaha, NE, USA
Publications:
An Unbiased Proteomics Method to Assess the Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes
- Cai W, Zhang J, de Lange WJ, Gregorich ZR, Karp H, Farrell ET, Mitchell SD, Tucholski T, Lin Z, Biermann M, McIlwain SJ, Ralphe JC, Kamp TJ, Ge Y. Circ Res. 2019 Nov 8;125(11):936-953. doi: 10.1161/CIRCRESAHA.119.315305. Epub 2019 Oct 1. PMID: 31573406; PMCID: PMC6852699
Proteomic Architecture of Human Coronary and Aortic Atherosclerosis
- Herrington DM, Mao C, Parker SJ, Fu Z, Yu G, Chen L, Venkatraman V, Fu Y, Wang Y, Howard TD, Jun G, Zhao CF, Liu Y, Saylor G, Spivia WR, Athas GB, Troxclair D, Hixson JE, Vander Heide RS, Wang Y, Van Eyk JE. 2018 Jun 19;137(25):2741-2756. doi: 10.1161/CIRCULATIONAHA.118.034365. PMID: 29915101; PMCID: PMC6011234
Splice-Junction-Based Mapping of Alternative Isoforms in the Human Proteome
- Lau E, Han Y, Williams DR, Thomas CT, Shrestha R, Wu JC, Lam MPY. Cell Rep. 2019 Dec 10;29(11):3751-3765.e5. doi: 10.1016/j.celrep.2019.11.026. PMID: 31825849; PMCID: PMC6961840
REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects
- Lee SH, Hadipour-Lakmehsari S, Murthy HR, Gibb N, Miyake T, Teng ACT, Cosme J, Yu JC, Moon M, Lim S, Wong V, Liu P, Billia F, Fernandez-Gonzalez R, Stagljar I, Sharma P, Kislinger T, Scott IC, Gramolini AO. Nat Commun. 2020 Feb 19;11(1):965. doi: 10.1038/s41467-019-14143-9. PMID: 32075961; PMCID: PMC7031342
Multi-omics of a pre-clinical model of diabetic cardiomyopathy reveals increased fatty acid supply impacts mitochondrial metabolic selectivity
- Li DK, Smith LE, Rookyard AW, Lingam SJ, Koay YC, McEwen HP, Twigg SM, Don AS, O'Sullivan JF, Cordwell SJ, White MY. J Mol Cell Cardiol. 2022 Mar;164:92-109. doi: 10.1016/j.yjmcc.2021.11.009. Epub 2021 Nov 24. PMID: 34826416
Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover
- Martin TG, Myers VD, Dubey P, Dubey S, Perez E, Moravec CS, Willis MS, Feldman AM, Kirk JA. Nat Commun. 2021 May 19;12(1):2942. doi: 10.1038/s41467-021-23272-z. PMID: 34011988; PMCID: PMC8134551
Myofilament glycation in diabetes reduces contractility by inhibiting tropomyosin movement, is rescued by cMyBPC domains
- Papadaki M, Kampaengsri T, Barrick SK, Campbell SG, von Lewinski D, Rainer PP, Harris SP, Greenberg MJ, Kirk JA. J Mol Cell Cardiol. 2022 Jan;162:1-9. doi: 10.1016/j.yjmcc.2021.08.012. Epub 2021 Sep 3. PMID: 34487755; PMCID: PMC8766917
Bottom-up proteomic analysis of human adult cardiac tissue and isolated cardiomyocytes
- Wojtkiewicz M, Berg Luecke L, Castro C, Burkovetskaya M, Mesidor R, Gundry RL. J Mol Cell Cardiol. 2022 Jan;162:20-31. doi: 10.1016/j.yjmcc.2021.08.008. PMID: 34437879
Integrated omics dissection of proteome dynamics during cardiac remodeling
- Lau E, Cao Q, Lam MPY, Wang J, Ng DCM, Bleakley BJ, Lee JM, Liem DA, Wang D, Hermjakob H, Ping P. Nat Commun. 2018 Jan 9;9(1):120. doi: 10.1038/s41467-017-02467-3. PMID: 29317621; PMCID: PMC5760723