Antibody resource pillar
Chair: Dr Cecilia Lindskog (Uppsala University, Sweden)
Overview of the HAI Project
The mission of the Human Antibody Pillar is to promote, and lead efforts using antibody-based strategies to explore and analyze the human proteome and the integration of such efforts with other initiatives of the Human Proteome Project (HPP). This mission is to disseminate appropriate approaches for validating antibodies used in common research and to promote guidelines to ensure reproducibility of antibody-based data.
The pillar consists of several separate activities including;
(1) The generation of an open access catalogue of validated antibodies from many different sources and for a variety of immunoassays. The Antibody Resource database (www.antibodypedia.org) has been set-up to provide an open access to a comprehensive catalogue of publicly available antibodies towards human proteins.
(2) Efforts to use validated antibodies to annotate all human protein-coding genes with a main emphasis on the “missing proteins” that have not yet been confirmed experimentally. A summary of the protein evidence for all protein-coding genes will be updated on an annual basis.
(3) Integration of antibody-based strategies and other omics technologies in the field of plasma proteomics in collaboration with HPP’s Plasma Proteome Project.
(4) Integration of antibody-based strategies and other omics technologies in the field of brain proteomics in collaboration with the HPP’s Brain Proteome Project
(5) Integration of antibody-based strategies and other omics technologies in the field of cancer research in collaboration with the HPP’s Pathology Pillar.
Image: Olfactory bulb from the Human Protein Atlas program
International Working Group for Antibody Validation
An ad hoc International Working Group for Antibody Validation, including participants from the HUPO Antibody Initiative, was formed to formulate appropriate approaches for validating antibodies used in common research applications, and to provide guidelines to ensure antibody reproducibility. The working group recommend five conceptual ‘pillars’ for antibody validation to be used in an application-specific manner, as outlined in the figure below.
The recommendations are summarized in the figure below and in the publication “A proposal for antibody validation”, Uhlén et al. Nature Methods. 2016 Sept 5.