" We are looking for mass spectrometry evidence of missing proteins (MPs) in chromosome 9. Our group focuses on identifing the molecular and cellular roles of MPs by using halo tag purification system and imaging technology"
PI : Je-Yoel CHO
We have been trying to reveal biological functions of MPs using human cell line models and immunoprecipitation-mass spectrometry (IP-MS) approach. This strategy is not only useful for neXt-CP50 uPE1 functional characterization project, but also next-MP50 MPs identification and validation project. Chromosome 9 contains a total of 103 missing protein candidates, and we focused on five proteins (FOXD4, ARID3C, OR1J1, ANKRD18A, and ZNF510). We constructed HaloTag tagged fusion protein expression vectors which expressed in mammalian cells to identify MPs’ subcellular localization and their binding partners.